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Figure 3. Memory of inflammatory injury features retention of H3K4me1 and manifests as an incomplete cell fate decision (A) Signal intensity traces and tornado plots of the histone modifications H3K4me1, <t>H3K27ac,</t> and H3K27me3 generated with CUT&TAG data at clusters 1/2/3/4 (as in Figure 1F).
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Figure 3. Memory of inflammatory injury features retention of H3K4me1 and manifests as an incomplete cell fate decision (A) Signal intensity traces and tornado plots of the histone modifications H3K4me1, <t>H3K27ac,</t> and H3K27me3 generated with CUT&TAG data at clusters 1/2/3/4 (as in Figure 1F).
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Figure 3. Memory of inflammatory injury features retention of H3K4me1 and manifests as an incomplete cell fate decision (A) Signal intensity traces and tornado plots of the histone modifications H3K4me1, <t>H3K27ac,</t> and H3K27me3 generated with CUT&TAG data at clusters 1/2/3/4 (as in Figure 1F).
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Figure 3. Memory of inflammatory injury features retention of H3K4me1 and manifests as an incomplete cell fate decision (A) Signal intensity traces and tornado plots of the histone modifications H3K4me1, <t>H3K27ac,</t> and H3K27me3 generated with CUT&TAG data at clusters 1/2/3/4 (as in Figure 1F).
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Figure 3. Memory of inflammatory injury features retention of H3K4me1 and manifests as an incomplete cell fate decision (A) Signal intensity traces and tornado plots of the histone modifications H3K4me1, <t>H3K27ac,</t> and H3K27me3 generated with CUT&TAG data at clusters 1/2/3/4 (as in Figure 1F).
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Figure 3. Memory of inflammatory injury features retention of H3K4me1 and manifests as an incomplete cell fate decision (A) Signal intensity traces and tornado plots of the histone modifications H3K4me1, <t>H3K27ac,</t> and H3K27me3 generated with CUT&TAG data at clusters 1/2/3/4 (as in Figure 1F).
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Figure 3. Memory of inflammatory injury features retention of H3K4me1 and manifests as an incomplete cell fate decision (A) Signal intensity traces and tornado plots of the histone modifications H3K4me1, <t>H3K27ac,</t> and H3K27me3 generated with CUT&TAG data at clusters 1/2/3/4 (as in Figure 1F).
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Figure 3. Memory of inflammatory injury features retention of H3K4me1 and manifests as an incomplete cell fate decision (A) Signal intensity traces and tornado plots of the histone modifications H3K4me1, <t>H3K27ac,</t> and H3K27me3 generated with CUT&TAG data at clusters 1/2/3/4 (as in Figure 1F).
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Figure 3. Memory of inflammatory injury features retention of H3K4me1 and manifests as an incomplete cell fate decision (A) Signal intensity traces and tornado plots of the histone modifications H3K4me1, <t>H3K27ac,</t> and H3K27me3 generated with CUT&TAG data at clusters 1/2/3/4 (as in Figure 1F).
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Figure 3. Memory of inflammatory injury features retention of H3K4me1 and manifests as an incomplete cell fate decision (A) Signal intensity traces and tornado plots of the histone modifications H3K4me1, <t>H3K27ac,</t> and H3K27me3 generated with CUT&TAG data at clusters 1/2/3/4 (as in Figure 1F).
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Figure 3. Memory of inflammatory injury features retention of H3K4me1 and manifests as an incomplete cell fate decision (A) Signal intensity traces and tornado plots of the histone modifications H3K4me1, <t>H3K27ac,</t> and H3K27me3 generated with CUT&TAG data at clusters 1/2/3/4 (as in Figure 1F).
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Figure 3. Memory of inflammatory injury features retention of H3K4me1 and manifests as an incomplete cell fate decision (A) Signal intensity traces and tornado plots of the histone modifications H3K4me1, <t>H3K27ac,</t> and H3K27me3 generated with CUT&TAG data at clusters 1/2/3/4 (as in Figure 1F).
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Figure 3. Memory of inflammatory injury features retention of H3K4me1 and manifests as an incomplete cell fate decision (A) Signal intensity traces and tornado plots of the histone modifications H3K4me1, H3K27ac, and H3K27me3 generated with CUT&TAG data at clusters 1/2/3/4 (as in Figure 1F).

Journal: Cell reports. Medicine

Article Title: Antigen-specific T cell responses in SARS-CoV-2 mRNA-vaccinated children.

doi: 10.1016/j.xcrm.2023.101298

Figure Lengend Snippet: Figure 3. Memory of inflammatory injury features retention of H3K4me1 and manifests as an incomplete cell fate decision (A) Signal intensity traces and tornado plots of the histone modifications H3K4me1, H3K27ac, and H3K27me3 generated with CUT&TAG data at clusters 1/2/3/4 (as in Figure 1F).

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2

Techniques: Generated